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Recombinant Human IGF-1 (1-70)

Derived from E. coli

Technical Specifications

Description

  • Recombinant Human Insulin-like Growth Factor 1 (IGF-1) is produced with our E. coli expression system. The target protein is expressed with sequence (G49-A118) of Human IGF-1.

  • Accession #: P05019

  • Synonyms: Insulin-Like Growth Factor 1, IGF-1, Mechano Growth Factor, MGF, Somatomedin-C, IGF-1,IBP-1

Formulation

Lyophilized from a 0.2 ?M filtered solution of 20mM acetic acid, 4% Mannitol,pH 4.5

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage

Reconstituted protein should be stored in a standard home refrigerator at 2°C to 8°C (35°F to 46°F) before and after opening. Before opening, vials are stable for up to six months, and after opening, vials are stable for 28 days at these temperatures.

All storage temperatures consistent with standard residential frost-free refrigerators/freezers.

Reconstitution

  • Always centrifuge tubes before opening. Do not mix by vortex pipetting.
  • It is not recommended to reconstitute to a concentration of less than 100?g/ml. Dissolve the lyophilized protein in 1X PBS.
  • Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Quality Control

  • Bioactivity*: The ED50 for this effect is typically 0.2-1.0 ng/ml. Corresponding to a specific activity of ? 1.0× 10^6 unit/mg.

  • *Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells.

  • Purity: Greater than 95% as determined by reducing SDS-PAGE.

  • Endotoxin: Less than 0.1 ng/?g (1 IEU/?g).

Amino Acid Sequence

GPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPA KSA

Background

Insulin-like growth factor 1 (IGF-1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids with 94% identity with mouse IGF-1 and exhibits cross-species activity. IGF-1 binds IGF-1R, IGF-2R, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone.

SDS Page

IGF1 Description
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